| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Melchore, Anthony F (14)
|
MRLIAC |
25.61 |
24.06 |
-1.55 |
| 1 |
O'Sullivan, Oisin (13)
|
MRSSC |
25.22 |
25.23 |
0.01 |
| 2 |
Olsen, Grady I (13)
|
MRCSC |
25.08 |
24.22 |
-0.86 |
| 2 |
Cornish, Kyle F (14)
|
MRLBA |
25.23 |
25.29 |
0.06 |
| 3 |
Keane, Konall (14)
|
MRCSC |
25.76 |
24.98 |
-0.78 |
| 3 |
Oh, Daniel (14)
|
MRHYB |
25.79 |
25.36 |
-0.43 |
| 4 |
Dowd, Michael R (13)
|
MRCSC |
25.77 |
25.43 |
-0.34 |
| 5 |
Liberato, Andrew (14)
|
MRIA |
25.86 |
25.59 |
-0.27 |
| 6 |
Konarski, Patrick K (14)
|
MRIA |
25.80 |
25.66 |
-0.14 |
| 7 |
Kang, Matthew (14)
|
MRBBSC |
25.11 |
25.69 |
0.58 |
| 8 |
Pergan III, Chester J (13)
|
MRTVSC |
26.35 |
25.71 |
-0.64 |
| 8 |
Phillip, Bryon J (14)
|
MRLIAC |
25.22 |
25.71 |
0.49 |
| 10 |
Tack, Lucas S (13)
|
MRTS |
25.94 |
25.76 |
-0.18 |
| 11 |
Lopez, Justen J (13)
|
MRLIAC |
26.07 |
25.79 |
-0.28 |
| 12 |
Nazareno, Andrew L (14)
|
MRBBSC |
25.72 |
25.89 |
0.17 |
| 13 |
Hwang, Colin (13)
|
MRLIAC |
25.59 |
25.93 |
0.34 |
| 14 |
Basak, Ronshekhar (14)
|
MRLIAC |
25.71 |
25.94 |
0.23 |
| 15 |
Wone, Brandon M (13)
|
MRSSC |
25.50 |
25.96 |
0.46 |
| 16 |
Lim, Hans Tristan C (13)
|
MRLIAC |
26.15 |
25.99 |
-0.16 |
| 17 |
Shopis, Nicholas (13)
|
MRLIAC |
25.20 |
26.00 |
0.80 |
| 18 |
Gingrass, Zachary K (13)
|
MRLIAC |
25.84 |
26.07 |
0.23 |
| 19 |
Shemet, Henry M (14)
|
MRTVSC |
26.58 |
26.08 |
-0.50 |
| 20 |
Todd, Ian (14)
|
MRHYB |
25.99 |
26.23 |
0.24 |
| 21 |
Yeung, Timothy (13)
|
MRLIAC |
26.55 |
26.35 |
-0.20 |
| 22 |
Davis, Avery J (14)
|
MRSSC |
26.75 |
26.36 |
-0.39 |
| 23 |
Orehosky, Bailey T (14)
|
MRHAA |
26.59 |
26.38 |
-0.21 |
| 24 |
Pineda, Kevin S (13)
|
MREHY |
26.26 |
26.42 |
0.16 |
| 25 |
McLaughlin, Joseph W (13)
|
MRBBSC |
26.01 |
26.53 |
0.52 |
| 26 |
Combs, Sean A (14)
|
MRSSC |
26.24 |
26.62 |
0.38 |